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Projects

LAMP-based digital quantification of HIV and HTLV for users at the point of care (Light-Up)

Projektbild

Project description

Within the project LIGHT-UP, a centrifugal microfluidic (LabDisk) platform for automated processing of digital amplification of HIV- and HTLV-RNA via LAMP (loop-mediated isothermal amplification) from a blood sample was developed. The LabDisk is applied for the measurement of the viral load to monitor the patient’s response to ART treatment. LIGHT-UP uses finger-prick samples instead of venous blood. Absolute quantification of HIV- and HTLV-RNA from the blood sample is reached within 2 hours. For the simultaneous detection of several targets in one reaction, LAMP was combined with a probe-based detection using the IMTEK-proprietary mediator displacement technology. The entire process flow from the blood sample take up to the absolut quantitative viral load result is integrated on the centrifugal-microfluidic LabDisk. The LIGHT-UP system is capable of analyzing HIV-/HTLV-patient samples directly at the point-of-care without the delay and logistic effort caused by transport of the sample to a central laboratory.

Start/End of project

01.02.2016 until 31.07.2019

Project manager

Lisa Becherer (Prof. Dr. Roland Zengerle)

Contact person

Lisa Becherer
Phone:73227

Partners

Mast Diagnostica GmbH, Max von Pettenkofer Institute, ELT Werkzeugbau GmbH, Datamed srl.

Funding

Bundesministerium für Bildung und Forschung / Federal Ministry of Education and Research

Keywords

Lab-on-a-Chip, Bioanalytik, Biosensoren, Analytik & Diagnostik, lab-on-a-chip, bioanalysis, biosensors, analytics & diagnostics
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