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SPEEDER project

    SPEEDER


    Abstract: Electrical fields are recognised as important guidance cues in the development and life cycle of human tissues. Many cells are to some extent electrotactic, meaning their migration can be controlled by an electrical field. During injury to the skin this happens naturally, when endogenous fields are generated due to the different ionic exchange on the intact part of the skin and the wound centre. In SPEEDER we investigate whether exogenously applied electrical fields can benefit from the same mechanism, to support healing of complicated wounds.

     

    https://www.imtek.de/nachwuchsgruppen/asplund/SPEEDERpics/woundef/ https://www.imtek.de/nachwuchsgruppen/asplund/SPEEDERpics/woundef/view

    SPEEDER concept

    In SPEEDER we use the conducting polymer poly (3,4-ethylene dioxythiophene) (PEDOT) to apply electrical fields. By developing supercapacitive versions of PEDOT, we are able to support a stable ionic current flow over time frames long enough to drive electrotaxis in cells, and to recharge electrodes in situ without reversing migration. As we can deliver direct current without corrosion, and our technology can work completely free from metals, our technology enables the transfer from petri dish to wound healing patch.

    SPEEDER update

    We are happy to announce that we have just published a proof of priciple in the prestigeous journal Biomaterials. We have shown that cells cultured in microfluidic devices can be stimulated with the SPEEDER electrodes and that the stimulation acts to direct the cell migration. The full text paper by José Leal can be downloaded open Access. Furthermore we culture epithelial cells to create model skin which we wound by making a scratch in the confluent cell layer. Then we use SPEEDER electrodes to study how the applied stimulation influence healing speed. Some exemplary data on these experiments you can already view on youtube

     

    SPEEDER publications

    • J. Leal-Ordonez, N. Jedrusik, S. Shaner, C. Boehler, M. Asplund*, “SIROF stabilized PEDOT/PSS allows biocompatible and reversible direct current stimulation capable of driving electrotaxis in cells”, Biomaterials, 2021, 7;275:120949. doi: 10.1016/j.biomaterials.
    • J. Leal, N. Jedrusik, S.Shaner, C. Boehler and M. Asplund 'Biocompatible and sustainable DC stimulation of cells and tissue based on highly swollen polymer electrodes', World Biomaterials Congress WBC-2020, held online in Dec 2020. Download poster here. 
    • C. Boehler, S Carli, L. Fadiga, T Stieglitz and Asplund M, "Tutorial: Guidelines for standardized performance tests for electrodes intended for neural interfaces and bioelectronics”, Nature Protocols,15, p. 3557–3578, 2020,  doi:10.1038/s41596-020-0389-2 >LINK<
    • S. Shaner, M. Islam, J. Korvink, M. Asplund, "Supercapactive polymer hydrogel electrode via pure PEDOT:PSS hydrogel printed on laser-induced graphene for DC stimulation applications," MRS Spring Meeting 2020, held online Dec 2020. Download abstract here
    • J. L. Ordonez, M. Dürr, N. Jedrusik and M. Asplund, "New Approach to Electrotaxis Experiments Utilizing Polyimide-Based PEDOT Electrodes in a PDMS Microfluidic Chip", MRS Spring Meeting, Phoenix, USA, April 23, 2019, July, 2018. >LINK<
    • C. Boehler, A. Schopf, J. Leal and M. Asplund, "Applications of PEDOT in bioelectronic medicine." Bioelectronics in Medicine, 2(2), 2019: 89–99, doi: 10.2217/bem-2019-0014. >LINK<
       
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    https://www.imtek.de/nachwuchsgruppen/asplund/SPEEDERpics/erclogo

 

     

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