Projects

Currently available methods for specific multiplex nucleic acid detection are whether restricted to around 6 targets that can be detected in parallel or require the laborious production of sequence-specific nucleic acid microarrays. The Mediator Probe Assay circumvents these bottlenecks by combination of nucleic acid amplification and detection of sequence-specific Mediators on a universal microarray of detection oligonucleotides. These assays combine the following steps in a closed reaction cartridge: Multiplex amplification of nucleic acid sequences to be detected, hybridization of gene-specific sequences to the target sequencem cleavage of Mediators off the hybridized probe, array-based detection of the Mediators. Mediators are standard oligonucleotides comprising a complementary sequence according to the detection array. The Mediator Probe Assay will be demonstrated in a PCR-based and an isothermal amplification based approach. The concept will be demonstrated by the detection of respiratory viruses. Beyond, the possibility of differentiated detection of genes and their transcripts is to be demonstrated. For more technological information, please see link below.