Light-Sheet Microscopy with Bessel beams and STED
Background and Problem: The extended ring system of the Bessel beam is required for self-re-construction, but excites fluores-cence inside the object above and below the focal plane and thus reduces image contrast. Approach 1: Using femto-second lasers, fluorescence inside the strongly scattering medium is excited by a two-photon process, leading to strong suppression of the unwanted fluorescence in the ring system. Approach 2: The principle of stimulated emission by depletion (STED) is applied for the first time to self-reconstructing beams. The un-wanted fluorescence in the Bessel beam’s ring system is depleted by a second self-reconstructing Bessel beam with a helical phase (charge number L = 3). |
Figures
Propagation of a two photon Bessel beam through a cluster of spheres. Scanning a two photon Bessel beam through a cluster of spheres. |
Publications
- Meinert T, Rohrbach A
Light-sheet microscopy with adaptive Bessel beams
2019 Biomed Opt Express, Band: 10, Nummer: 2, Seiten: 670 - 681 - Bhat A, Meinert T, Michiels R, Rohrbach A
Miniature scanning light-sheet illumination implemented in a conventional microscope
2018 Biomed Opt Express, Band: 9, Nummer: 9, Seiten: 4263 - 4274 - Gohn-Kreuz C, Rohrbach A
Light needles in scattering media using self-reconstructing beams and the STED principle
2017 Optica, Band: 4, Nummer: 9, Seiten: 1134 - 1142 - Meinert T, Gutwein B-A, Rohrbach A
Light-sheet microscopy in a glass capillary – Feedback holographic control for illumination beam correction
2016 Opt Lett, Band: 42, Nummer: 2, Seiten: 350 - 353 - Meinert T, Tietz O, Palme K, Rohrbach A
Separation of ballistic and diffusive fluorescence photons in confocal Light-Sheet Microscopy of Arabidopsis roots
2016 Sci Rep, Band: 6, Seite: 30378 - Gohn-Kreuz C, Rohrbach A
Light-sheet generation in inhomogeneous media using self-reconstructing beams and the STED-principle
2016 Opt Express, Band: 24, Nummer: 6, Seiten: 5855 - 5865 - Mousavi H, Gohn-Kreuz C, Rohrbach A
Feedback phase correction of Bessel beams in confocal line light-sheet microscopy – a simulation study
2013 Appl Optics, Band: 52, Nummer: 23, Seiten: 5835 - 5842 - Fahrbach F, Gurchenkov V, Alessandri K, Nassoy P, Rohrbach A
Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation 2013 Opt Express, Band: 21, Nummer: 11, Seiten: 13824 - 13839 - Fahrbach F, Gurchenkov V, Alessandri K, Nassoy P, Rohrbach A
Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy
2013 Opt Express, Band: 21, Nummer: 9, Seiten: 11425 - 11440 - Fahrbach F, Rohrbach A
Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media 2012 Nat Commun, Band: 3, Seite: 632 - F. O. Fahrbach, A. Rohrbach
A line scanned light-sheet microscope with phase shaped self-reconstructing beams
2010 Optics Express, Band: 18, Nummer: 23, Seiten: 24229 - 24244 - F. O. Fahrbach, P. Simon, A. Rohrbach
Microscopy with self-reconstructing beams
2010 Nature Photonics, Band: 4, Seiten: 780 - 785 - Rohrbach A
Artifacts resulting from imaging in scattering media: a theoretical prediction.
2009 Opt Lett, Band: 34, Nummer: 19, Seiten: 3041 - 3043